Part:BBa_M45091:Design
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Saccharomyces Cerevisie.
Efficient mating requires stable contact between the two partners and is facilitated by mating type specific cell
surface glycol proteins, called 'a' and 'α' agglutinins. Both agglutinins are induced by exposure of cells to the
pheromone by cells of opposite mating types. AGA1 RNA is expressed in both 'a' and 'α' cells after pheromone
induction (Nobel et al, 1995).
The a-agglutinin consists of 73 KDa cell wall proteins, the product of AGA-gene, and a disulfide-linked small
peptide (63 amino acid). The a-agglutinin molecule consists of up to 30% seryl and threonyl residues almost all of
which seem to be linked to saccharides. A central region (amino acid 27-42) is free hydroxyl amino acid and is
considered as a potential binding region. This indicates that the attachment of a-agglutinin to some cell wall
components. Thus a-agglutinin is attached via two sulfide linkages to a cell wall component, in all likelihood to
the gene product of AGA1 - an extremely serine/threonine rich protein (Capellaro et al. 1994).
In this project AGA1
gene is obtained from NCBI (NM_001183221.1) from the website and is amplified by PCR.
When 'a'-cell are treated with either mercaptoethanol or with dithiothreitol, the, the cell responsible for sexual agglutination is easily lost to the medium.
Source
NCBI Reference Sequence: NM_001183221.1
References
Hans de Nobel, Jeremy Pike, Peter N. Lipke, Janet Kurjan. Genetics of a-agglutunin function in Saccharomyces cerevisiae. Molecular and General Gen Genet 1995, 247: 409-415.
Corinna Cappellaro, Cornelia Baldermann, Reinhard Rache, Widmar Tanner. Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and x-agglutinin1. The EMBO Journal 1994 4737-4744.